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Amavuta meza ya Organic Lavender Amavuta yingenzi yo kuvura uruhu rwa Aromatherapy
Uburyo bwo kuvoma cyangwa gutunganya: Amashanyarazi
Gukuramo Disillation Igice: Indabyo
Inkomoko y'igihugu: Ubushinwa
Gusaba: Diffuse / aromatherapy / massage
Ubuzima bwa Shelf: 3years
Serivise yihariye: ikirango cyihariye nagasanduku cyangwa nkuko ubisabwa
Icyemezo: GMPC / FDA / ISO9001 / MSDS / COA
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100% Byiza kama Organic Magnoliae Officmalis Cortex Amavuta Yingenzi Yokwitaho Uruhu
Impumuro ya Hou Po ihita isharira kandi irakaze cyane noneho ifungura buhoro buhoro uburyohe bwimbitse, sirupi n'ubushyuhe.
Hou Po ifitanye isano nisi nibintu byuma aho ubushyuhe bukabije bukora cyane kumanuka Qi nubushuhe bwumye. Kubera iyo mico, ikoreshwa mubuvuzi bwubushinwa kugirango igabanye guhagarara no kwirundanyiriza mu nzira yigifu kimwe no gukorora no gutontoma bitewe na flegm ibuza ibihaha.
Magnolia Officinials ni igiti cyimeza kiva mu misozi n'ibibaya bya Sichuan, Hubei n'izindi ntara z'Ubushinwa. Igishishwa cyiza cyane gikoreshwa mubuvuzi gakondo bwabashinwa bakuwe mubiti, amashami n'imizi Byegeranijwe muri Mata kugeza muri Kamena. Igishishwa cyijimye, cyoroshye, kiremereye hamwe namavuta, gifite ibara ryumutuku kuruhande rwimbere hamwe na kirisiti nka sheen.
Abimenyereza barashobora gutekereza guhuza Hou Po na Qing Pi amavuta yingenzi nkinoti yo hejuru ishimirwa mubuvange bugamije gusenya ibintu.
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OEM Custom Package Kamere ya Macrocephalae Amavuta ya Rhizoma
Nkumuti mwiza wa chimiotherapeutique, 5-fluorouracil (5-FU) ikoreshwa cyane mukuvura ibibyimba bibi mumitsi yigifu, umutwe, ijosi, igituza, nintanga ngore. Kandi 5-FU numuti wambere kumurongo wa kanseri yibara mumavuriro. Uburyo bwibikorwa bya 5-FU nuguhagarika ihinduka rya acide nucleic acide ya acide ya thymine nucleic acide yibibyimba, hanyuma bikagira ingaruka kuri synthesis no gusana ADN na RNA kugirango bigere ku ngaruka za cytotoxic (Afzal et al., 2009; Ducreux et al., 2015; Longley n'abandi, 2003). Nyamara, 5-FU itanga kandi chimiotherapie iterwa n'impiswi (CID), imwe mu ngaruka mbi zikunze kwibasira abarwayi benshi (Filho et al., 2016). Umubare w'impiswi ku barwayi bavuwe na 5-FU wageze kuri 50% - 80%, ibyo bikaba byaragize ingaruka zikomeye ku iterambere n'imikorere ya chimiotherapie (Iacovelli et al., 2014; Rosenoff et al., 2006). Kubwibyo, ni ngombwa cyane gushakisha uburyo bwiza bwo kuvura 5-FU iterwa na CID.
Kugeza ubu, ibiyobyabwenge bidafashwe n’ibiyobyabwenge byinjijwe mu buvuzi bwa CID. Ibikorwa bitarimo ibiyobyabwenge birimo indyo yuzuye, no kongeramo umunyu, isukari nintungamubiri. Ibiyobyabwenge nka loperamide na octreotide bikoreshwa cyane mu kuvura anti-diarrhea ivura CID (Benson et al., 2004). Byongeye kandi, Ethnomedicines nayo yemewe kuvura CID hamwe nubuvuzi bwabo bwihariye mubihugu bitandukanye. Ubuvuzi gakondo bw'Abashinwa (TCM) ni bumwe mu bwoko bwa Ethnomedicine bumaze imyaka irenga 2000 bukorwa mu bihugu bya Aziya y'Uburasirazuba harimo Ubushinwa, Ubuyapani na Koreya (Qi et al., 2010). TCM ivuga ko imiti ya chimiotherapeutique yatera Qi kurya, kubura intanga, kutagira igifu no kugabanuka kwa endofitike, bikaviramo gukora nabi amara. Mu nyigisho za TCM, ingamba zo kuvura CID zigomba ahanini gushingira ku kuzuza Qi no gukomeza ururenda (Wang et al., 1994).
Imizi yumye yaAtractylode macrocephalaKoidz. (AM) naPanax ginsengCA Mey. (PG) ni imiti isanzwe y'ibyatsi muri TCM hamwe n'ingaruka zimwe zo kuzuza Qi no gukomeza ururenda (Li et al., 2014). AM na PG mubisanzwe bikoreshwa nkibimera (uburyo bworoshye bwo guhuza ibimera byabashinwa) hamwe ningaruka zo kuzuza Qi no gukomeza ururenda rwo kuvura impiswi. Kurugero, AM na PG byanditswe muburyo bwa kera bwo kurwanya impiswi nka Shen Ling Bai Zhu San, Si Jun Zi Tang kuvaTaiping Huimin Heji Ju Fang(Ingoma yindirimbo, Ubushinwa) na Bu Zhong Yi Qi Tang kuvaPi Wei Lun(Ingoma ya Yuan, Ubushinwa) (Ishusho 1). Ubushakashatsi bwinshi bwibanze bwari bwaravuze ko formula zose uko ari eshatu zifite ubushobozi bwo kugabanya CID (Bai et al., 2017; Chen et al., 2019; Gou et al., 2016). Byongeye kandi, ubushakashatsi bwatubanjirije bwerekanye ko Shenzhu Capsule irimo AM na PG gusa igira ingaruka zishobora kuvura imiti yimpiswi, kolite (syndrome ya xiexie), nizindi ndwara zifata igifu (Feng et al., 2018). Ariko, nta bushakashatsi bwigeze buganira ku ngaruka nuburyo bwa AM na PG mu kuvura CID, haba hamwe cyangwa wenyine.
Noneho amara microbiota afatwa nkikintu gishobora gusobanukirwa uburyo bwo kuvura TCM (Feng et al., 2019). Ubushakashatsi bugezweho bwerekana ko microbiota yo munda igira uruhare runini mukubungabunga amara homeostasis. Microbiota nzima igira uruhare mukurinda amara, metabolisme, immunostasis yumubiri nigisubizo, hamwe no guhagarika indwara (Thursdayby na Juge, 2017; Pickard et al., 2017). Indwara ya microbiota idahwitse yangiza imikorere yumubiri nubudahangarwa bwumubiri wumuntu muburyo butaziguye cyangwa butaziguye, bitera ingaruka zimpiswi nka diarrhea (Patel et al., 2016; Zhao na Shen, 2010). Ubushakashatsi bwerekanye ko 5-FU yahinduye ku buryo budasanzwe imiterere ya microbiota yo mu nda mu mbeba za diarrheic (Li et al., 2017). Kubwibyo, ingaruka za AM na PM kuri 5-FU zatewe nimpiswi zirashobora guhuzwa na microbiota yo munda. Ariko, niba AM na PG byonyine kandi hamwe bishobora gukumira 5-FU iterwa nimpiswi muguhindura mikorobe yo mu nda ntikiramenyekana.
Kugirango dukore iperereza ku ngaruka zo kurwanya impiswi hamwe nuburyo bwibanze bwa AM na PG, twakoresheje 5-FU twigana urugero rwimpiswi mu mbeba. Hano, twibanze ku ngaruka zishobora guterwa nubuyobozi bumwe kandi buhuriweho (AP) bwaAtractylode macrocephalaamavuta ya ngombwa (AMO) naPanax ginsengsaponine yuzuye (PGS), ibice bikora byakuwe muri AM na PG, kumpiswi, indwara zo munda hamwe na mikorobe nyuma ya chimiotherapie 5-FU.
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100% Amavuta meza ya Eucommiae Foliuml Amavuta Yingenzi Yokwitaho Uruhu
Eucommia ulmoides(EU) (bakunze kwita “Du Zhong” mu rurimi rw'igishinwa) ni umuryango wa Eucommiaceae, ubwoko bw'igiti gito kavukire mu Bushinwa bwo hagati [1]. Iki gihingwa gihingwa cyane mu Bushinwa ku rugero runini kubera akamaro k’ubuvuzi. Ibice bigera kuri 112 byatandukanijwe n’ubumwe bw’ibihugu by’Uburayi birimo lignans, iridoide, fenolike, steroid, n’ibindi bikoresho. Ibimera byiyongera byiki kimera (nkicyayi kiryoshye) cyerekanye imiti imwe n'imwe. Ikibabi cya EU gifite ibikorwa byinshi bijyanye na cortex, indabyo, n'imbuto [2,3]. Bivugwa ko amababi y’ubumwe bw’ibihugu by’Uburayi yongerera imbaraga amagufwa n'imitsi y'umubiri [4], bityo biganisha ku kuramba no guteza imbere uburumbuke mu bantu [5]. Ifu yicyayi iryoshye ikozwe mu kibabi cy’ubumwe bw’ibihugu by’Uburayi ngo yagabanije kubyibuha no kongera ingufu za metabolism. Ibikoresho bya Flavonoide (nka rutin, aside chlorogene, aside ferulike, na acide cafeque) byavuzwe ko byerekana ibikorwa bya antioxydants mu bibabi by’Uburayi [6].
Nubwo hari ibitabo bihagije byerekeranye na phytochemiki yumuryango w’ubumwe bw’ibihugu by’Uburayi, ubushakashatsi buke ariko bwabayeho ku miterere ya farumasi y’ibintu bitandukanye byakuwe mu bishishwa, imbuto, ibiti, n’ibibabi by’Uburayi. Uru rupapuro ruzasubiramo ruzasobanura amakuru arambuye yerekeranye n’ibintu bitandukanye byakuwe mu bice bitandukanye (ibishishwa, imbuto, uruti, n’ibabi) by’ubumwe bw’ibihugu by’Uburayi hamwe n’ikoreshwa ry’ibi bikoresho mu bintu biteza imbere ubuzima hamwe n'imirongo ya siyansi y'ibimenyetso bityo bigatanga ibikoresho bifatika. Kuri Porogaramu ya EU.
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Amavuta meza ya Houttuynia cordata amavuta Houttuynia Cordata Amavuta Lchthammolum
Mu bihugu byinshi bikiri mu nzira y'amajyambere, 70-95% by'abaturage bashingira ku miti gakondo yo kwita ku buzima bw'ibanze kandi muri 85% by'abantu bakoresha ibimera cyangwa ibiyikomokaho nk'ibintu bifatika.1Gushakisha ibinyabuzima bishya bikomoka ku bimera mubisanzwe biterwa namakuru yihariye y’amoko n’abaturage yabonetse kubimenyereza umwuga kandi biracyafatwa nkisoko yingenzi yo kuvumbura ibiyobyabwenge. Mu Buhinde, ibiyobyabwenge bigera ku 2000 bikomoka ku bimera.2] Urebye inyungu nyinshi zo gukoresha ibimera bivura, ubu bushakashatsi kuriHouttuynia cordataThunb. itanga amakuru agezweho yerekeranye na botaniki, ubucuruzi, etnofarmacologiya, phytochemiki na farumasi igaragara mubitabo.H. cordataThunb. ni iy'umuryangoSaururaceaekandi bizwi cyane nkumurizo wumushinwa. Ni ibyatsi bimera hamwe na rhizome ya stoloniferous ifite chemotypes ebyiri zitandukanye.3,4Imiti y’Abashinwa y’ubwoko iboneka mu gasozi ndetse n’igice cyo mu gasozi mu majyaruguru y’Uburasirazuba bw’Ubuhinde kuva muri Mata kugeza muri Nzeri.5,6,7]H. cordatairaboneka mu Buhinde, cyane cyane mu kibaya cya Brahmaputra cya Assam kandi ikoreshwa n'imiryango itandukanye ya Assam mu buryo bw'imboga ndetse no mu buvuzi butandukanye gakondo.
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100% Amavuta meza ya Lappa Yumukoresha - Amavuta ya Lime Arctium ya lappa hamwe nibyemezo byubuziranenge;
Inyungu zubuzima
Imizi ya burdock ikunze kuribwa, nyamara, irashobora kandi gukama no gushiramo icyayi. Ikora neza nkisoko ya inulin, aprebioticfibre ifasha igogora kandi itezimbere ubuzima bwinda. Byongeye kandi, iyi mizi irimo flavonoide (intungamubiri z’ibimera),phytochemicals, na antioxydants izwiho kugira ubuzima bwiza.
Mubyongeyeho, imizi ya burdock irashobora gutanga izindi nyungu nka:
Mugabanye umuriro udashira Umuzi wa Burdock urimo antioxydants nyinshi, nka quercetin, acide fenolike, na luteolin, zishobora gufasha kurinda selile zaweradicals yubuntu. Iyi antioxydants ifasha kugabanya gucana umubiri wose.
Ingaruka z'ubuzima
Imizi ya Burdock ifatwa nkumutekano kurya cyangwa kunywa nkicyayi. Nyamara, iki gihingwa gisa cyane na belladonna nighthade ibimera, bifite uburozi. Birasabwa kugura gusa imizi ya burdock kubagurisha bizewe no kwirinda kuyikusanya wenyine. Byongeye kandi, hari amakuru make ku ngaruka zayo ku bana cyangwa ku bagore batwite. Vugana na muganga wawe mbere yo gukoresha imizi ya burdock hamwe nabana cyangwa niba utwite.
Hano hari izindi ngaruka zishoboka zubuzima ugomba gusuzuma niba ukoresheje imizi ya burdock:
Kongera umwuma
Imizi ya Burdock ikora nka diuretique isanzwe, ishobora gutera umwuma. Niba ufashe ibinini byamazi cyangwa izindi diuretique, ntugomba gufata imizi ya burdock. Niba ufashe iyi miti, ni ngombwa kumenya indi miti, ibyatsi, nibindi bintu bishobora gutera umwuma.
Allergic
Niba ufite ibyiyumvo cyangwa ufite amateka ya allergique yatewe na dais, ragweed, cyangwa chrysanthemum, ushobora kuba ufite ibyago byinshi byo guhura na allergique kumuzi ya burdock.
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Igiciro kinini cyinshi 100% AsariRadix Et Rhizoma yamavuta Relax Aromatherapy Eucalyptus globulus
Ubushakashatsi ku nyamaswa no muri vitro bwakoze ubushakashatsi ku ngaruka zishobora gutera antifungal, anti-inflammatory, na cardiovascular ingaruka za sassafras n'ibiyigize. Nyamara, ibizamini byamavuriro birabuze, kandi sassafras ntabwo ifatwa nkumutekano kugirango ikoreshwe. Safrole, igice kinini cyibiti byumuzi wa sassafras hamwe namavuta, byabujijwe n’ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge (FDA), harimo no gukoresha uburyohe cyangwa impumuro nziza, kandi ntigomba gukoreshwa imbere cyangwa hanze, kuko gishobora kuba kanseri. Safrole yakoreshejwe mu bicuruzwa bitemewe na 3,4-methylene-dioxymethamphetamine (MDMA), izwi kandi ku mazina yo mu muhanda “ecstasy” cyangwa “Molly,” kandi igurishwa ry’amavuta ya safrole na sassafras rikurikiranwa n’ubuyobozi bushinzwe kurwanya ibiyobyabwenge muri Amerika.
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Igiciro cyinshi Igiciro 100% Cyiza Stellariae Radix amavuta yingenzi (mashya) Humura Aromatherapy Eucalyptus globulus
Pharmacopoeia yo mu Bushinwa (integuro ya 2020) isaba ko methanol ikuramo YCH itagomba kuba munsi ya 20.0% [2], nta bindi bipimo byerekana isuzumabumenyi byagaragaye. Ibyavuye muri ubu bushakashatsi byerekana ko ibikubiye muri methanol yakuwe mu gasozi no guhinga byombi byujuje ubuziranenge bwa farumasi, kandi nta tandukaniro rikomeye ryari hagati yabo. Kubwibyo, nta tandukanyirizo ryiza ryagaragaye hagati yintangarugero n’ibihingwa, ukurikije icyo cyerekezo. Nyamara, ibikubiye muri steroli zose hamwe na flavonoide zose murugero rwo mwishyamba byari hejuru cyane ugereranije nibihingwa. Ubundi isesengura rya metabolomic ryerekanye itandukaniro ryinshi rya metabolite hagati yinyamanswa n’ibihingwa. Byongeye kandi, 97 metabolite zitandukanye zitandukanye zerekanwe hanze, ziri kurutonde muriImbonerahamwe y'inyongera S2. Muri izi metabolite zitandukanye cyane harimo β-sitosterole (ID ni M397T42) hamwe n’ibikomoka kuri quercetin (M447T204_2), byavuzwe ko ari ibintu bikora. Mbere abatora batamenyekanye, nka trigonelline (M138T291_2), betaine (M118T277_2), fustin (M269T36), rotenone (M241T189), arctiin (M557T165) na aside loganike (M399T284_2), harimo na metabolite zitandukanye. Ibi bice bigira uruhare runini mukurwanya okiside, kurwanya inflammatory, gusibanganya radicals yubusa, kurwanya kanseri no kuvura ateriyose, bityo rero, bishobora kuba ibintu bishya byerekana ibintu bishya muri YCH. Ibiri mu bikoresho bikora bigena imikorere nubwiza bwibikoresho byimiti [7]. Muncamake, methanol ikuramo nkigipimo cyonyine cyo gusuzuma ubuziranenge bwa YCH gifite aho kigarukira, kandi nibindi bimenyetso byihariye bigomba gushakishwa. Hariho itandukaniro rikomeye muri steroli zose, flavonoide zose hamwe nibiri mubindi byinshi bitandukanya metabolite hagati yishyamba na YCH bihingwa; rero, hashobora kubaho itandukaniro ryiza hagati yabo. Muri icyo gihe, ibintu bishya byavumbuwe bishobora gukoreshwa muri YCH bishobora kugira agaciro gakomeye kokwiga ishingiro ryimikorere ya YCH hamwe no kurushaho guteza imbere umutungo wa YCH.
Akamaro k'ibikoresho by'imiti nyabyo bimaze kumenyekana mu karere kavukire ko gukora imiti y'ibyatsi yo mu Bushinwa ifite ubuziranenge buhebuje [8]. Ubwiza buhanitse ni ikintu cyingenzi cyibikoresho byubuvuzi nyabyo, kandi aho uba ni ikintu cyingenzi kigira ingaruka kumiterere yibyo bikoresho. Kuva YCH yatangira gukoreshwa nkubuvuzi, kuva kera yiganjemo YCH yo mu gasozi. Nyuma yo gutangiza no gutunga YCH muri Ningxia mu myaka ya za 1980, isoko y’ibikoresho by’imiti Yinchaihu yagiye ihinduka buhoro buhoro iva mu gasozi ijya YCH ihingwa. Nkurikije iperereza ryabanje kubyerekeye YCH [9] n'iperereza ryakozwe nitsinda ryacu ryubushakashatsi, hari itandukaniro rikomeye mubice byo gukwirakwiza ibikoresho bihingwa n’imiti yo mu gasozi. YCH yo mu gasozi ikwirakwizwa cyane mu karere ka Ningxia Hui yigenga mu Ntara ya Shaanxi, yegeranye n'akarere gakakaye ka Mongoliya y'imbere na Ningxia rwagati. By'umwihariko, ubutayu bwo mu butayu muri utwo turere ni ahantu heza ho gukura kwa YCH. Ibinyuranye na byo, YCH ihingwa ikwirakwizwa cyane cyane mu majyepfo y’ahantu ho gukwirakwiza amashyamba, nko mu Ntara ya Tongxin (Guhinga I) no mu turere tuyikikije, ikaba yarahindutse ikibanza kinini cyo guhingamo no gutanga umusaruro mu Bushinwa, ndetse n’intara ya Pengyang (Guhinga II) , iherereye mu majyepfo cyane kandi ni ahandi hantu habera umusaruro YCH ihingwa. Byongeye kandi, aho uturere twavuzwe haruguru twahinzwe ntabwo ari ubutayu. Kubwibyo, usibye uburyo bwo kubyaza umusaruro, hari kandi itandukaniro rikomeye mubituro byishyamba kandi bihingwa YCH. Imiturire ni ikintu cyingenzi kigira ingaruka kumiterere yibikoresho byimiti. Ahantu hatandukanye hazagira ingaruka ku miterere no kwegeranya metabolite ya kabiri mu bimera, bityo bikagira ingaruka ku bwiza bw’ibicuruzwa bivura imiti [10,11]. Kubwibyo, itandukaniro rigaragara mubirimo flavonoide yose hamwe na steroli zose hamwe no kwerekana metabolite 53 twasanze muri ubu bushakashatsi bishobora kuba ibisubizo byo gucunga imirima no gutandukanya aho batuye.Bumwe mu buryo bw'ingenzi ibidukikije bigira ingaruka ku bwiza bw’ibikoresho by’imiti ni uguhangayikisha ibimera bikomoka. Guhangayikisha ibidukikije bidakabije bikunda gutera imbaraga za metabolite ya kabiri [12,13]. Gukura / gutandukanya impirimbanyi hypothesis ivuga ko, iyo intungamubiri zihagije, ibimera bikura cyane cyane, mugihe iyo intungamubiri zibuze, ibimera ahanini bitandukanya kandi bikabyara metabolite ya kabiri [14]. Guhangayikishwa n’amapfa biterwa no kubura amazi nicyo kibazo nyamukuru cy’ibidukikije gihura n’ibimera ahantu humye. Muri ubu bushakashatsi, amazi y’ubuhinzi bwa YCH ni menshi cyane, hamwe n’imvura igwa buri mwaka iruta cyane iy'ishyamba rya YCH (amazi yo guhinga Ihinzwe I yikubye inshuro 2 iy'Ishyamba; Guhinga II yikubye inshuro 3,5 ubw'ishyamba ). Byongeye kandi, ubutaka mubidukikije ni ubutaka bwumucanga, ariko ubutaka bwo murima nubutaka bwibumba. Ugereranije n'ibumba, ubutaka bwumucanga bufite ubushobozi buke bwo gufata amazi kandi birashoboka cyane ko byongera amapfa. Muri icyo gihe, gahunda yo guhinga akenshi yajyanaga no kuvomera, bityo urugero rw’amapfa rukaba ruke. Ishamba rya YCH rikura ahantu habi hakeye, bityo rero rishobora guhura n’amapfa akomeye.Osmoregulation ni uburyo bwingenzi bwimiterere yibimera bihangayikishwa n amapfa, kandi alkaloide ningirakamaro ya osmotic igenga ibihingwa byo hejuru [15]. Betaine ni amazi ya elegitoronike ya alkaloide quaternary ammonium kandi irashobora gukora nka osmoprotectants. Guhangayikishwa n’amapfa birashobora kugabanya ubushobozi bwa osmotic selile, mugihe osmoprotectants ibungabunga kandi igakomeza imiterere nubusugire bwa macromolecules yibinyabuzima, kandi bikagabanya neza ibyangijwe n’amapfa yatewe n’ibimera [16]. Kurugero, mugihe cyamapfa, betaine yibisukari bya beterave na barisiyumu ya Lycium yiyongereye cyane [17,18]. Trigonelline ni igenzura ry'imikurire y'utugingo ngengabuzima, kandi mu gihe cy'amapfa, irashobora kwagura uburebure bw'utugingo ngengabuzima, ikabuza imikurire y'uturemangingo kandi bigatuma ingano ya selile igabanuka. Kwiyongera ugereranije no kwibumbira mu ngirabuzimafatizo bifasha igihingwa kugera ku mikorere ya osmotic no kongera ubushobozi bwo guhangana n’amapfa [19]. JIA X [20] yasanze, hamwe no kwiyongera kw’amapfa, Astragalus membranaceus (isoko y’ubuvuzi gakondo bw’Abashinwa) yabyaye trigonelline nyinshi, ikora mu kugenzura ubushobozi bwa osmotic no kunoza ubushobozi bwo guhangana n’amapfa. Flavonoide yerekanwe kandi ko igira uruhare runini mukurwanya ibimera kurwanya amapfa [21,22]. Umubare munini w’ubushakashatsi wemeje ko guhangayikishwa n’amapfa bitagereranywa byafashaga kwirundanya kwa flavonoide. Lang Duo-Yong n'abandi. [23] kugereranya ingaruka ziterwa n amapfa kuri YCH mugenzura ubushobozi bwo gufata amazi mumurima. Byagaragaye ko guhangayikishwa n’amapfa byabujije imikurire y’imizi ku rugero runaka, ariko mu gihe cy’amapfa yoroheje kandi akomeye (ubushobozi bwo gufata amazi yo mu murima 40%), flavonoide yuzuye muri YCH yariyongereye. Hagati aho, mu gihe cy’amapfa, phytosterole irashobora gukora kugirango igabanye ingirabuzimafatizo ya selile kandi itembera neza, ibuze gutakaza amazi no kunoza imihangayiko [24,25]. Kubwibyo, kwiyongera kwinshi kwa flavonoide, steroli zose, betaine, trigonelline nizindi metabolite ya kabiri muri YCH yo mu gasozi bishobora kuba bifitanye isano n’amapfa akomeye cyane.Muri ubu bushakashatsi, isesengura ryitunganyirizwa rya KEGG ryakozwe kuri metabolite wasangaga itandukanye cyane hagati yishyamba na YCH ihingwa. Metabolite ikungahaye cyane harimo n'abagize uruhare mu nzira ya asorbate na aldarate metabolism, aminoacyl-tRNA biosynthesis, metabolism ya histidine na beta-alanine metabolism. Izi nzira zo guhinduranya zifitanye isano rya bugufi nuburyo bwo kurwanya ibimera. Muri byo, metabolism ya ascorbate igira uruhare runini mu musaruro wa antioxydeant y’ibimera, metabolisme ya karubone na azote, kurwanya imihangayiko n’indi mirimo ya physiologiya [26]; aminoacyl-tRNA biosynthesis ninzira yingenzi yo gukora poroteyine [27,28], igira uruhare mu guhuza poroteyine zidashobora kwihanganira. Inzira zombi za histidine na β-alanine zirashobora kongera kwihanganira ibimera kwihanganira ibidukikije [29,30]. Ibi birerekana kandi ko itandukaniro rya metabolite hagati yishyamba n’ibihingwa bya YCH byari bifitanye isano rya bugufi nuburyo bwo kurwanya imihangayiko.Ubutaka ni ishingiro ryibintu bikura niterambere ryibiti bivura. Azote (N), fosifore (P) na potasiyumu (K) mu butaka ni intungamubiri zingenzi mu mikurire no gukura kw'ibimera. Ubutaka kama nubutaka burimo N, P, K, Zn, Ca, Mg nibindi macroelements hamwe nibintu bikenerwa kubiti bivura. Intungamubiri zirenze urugero cyangwa zidahagije, cyangwa igipimo cyintungamubiri zingana, bizagira ingaruka kumikurire niterambere ndetse nubwiza bwibikoresho bivura imiti, kandi ibimera bitandukanye bifite intungamubiri zitandukanye [31,32,33]. Kurugero, guhangayikishwa na N byateje imbere synthesis ya alkaloide muri Isatis indigotica, kandi byagize akamaro mukwirundanya kwa flavonoide mubihingwa nka Tetrastigma hemsleyanum, Crataegus pinnatifida Bunge na Dichondra repens Forst. Ibinyuranye na byo, N cyane yabujije kwirundanya kwa flavonoide mu moko nka Erigeron breviscapus, Abrus cantoniensis na Ginkgo biloba, kandi bigira ingaruka ku bwiza bw’ibikoresho by’imiti [34]. Gukoresha ifumbire ya P byagize akamaro mu kongera aside ya glycyrrhizic na dihydroacetone muri Ural licorice [35]. Iyo amafaranga yo gusaba yarenze 0 · 12 kg · m - 2, ibirimo flavonoide byose muri Tussilago farfara byagabanutse [36]. Gukoresha ifumbire ya P byagize ingaruka mbi kubiri muri polysaccharide mubuvuzi gakondo bwabashinwa rhizoma polygonati [37], ariko ifumbire ya K yagize akamaro mu kongera ibiyigize bya saponine [38]. Gukoresha kg 450 · hm - 2 K ifumbire yari nziza mu mikurire no kwirundanya kwa saponine yimyaka ibiri Panax notoginseng [39]. Ku kigereranyo cya N: P: K = 2: 2: 1, igiteranyo cy’ibikomoka kuri hydrothermal, harpagide na harpagoside byari byinshi cyane [40]. Umubare munini wa N, P na K wagize akamaro mu kuzamura imikurire ya cabine ya Pogostemon no kongera ibirimo amavuta ahindagurika. Umubare muto wa N, P na K wongereye ibikubiye mubintu byingenzi bigize Pogostemon cablin amavuta yibibabi [41]. YCH ni igihingwa cyihanganira ubutaka, kandi gishobora kuba gifite ibisabwa byihariye ku ntungamubiri nka N, P na K. Muri ubu bushakashatsi, ugereranije na YCH ihingwa, ubutaka bw’ibiti byo mu gasozi YCH bwari butarumbuka: ibirimo ubutaka. y'ibinyabuzima, byose hamwe N, byose hamwe na K byose hamwe byari hafi 1/10, 1/2, 1/3 na 1/3 cyibimera bihingwa. Kubwibyo, itandukaniro ryintungamubiri zubutaka rishobora kuba indi mpamvu yo gutandukanya metabolite yagaragaye muguhinga no mwishyamba YCH. Weibao Ma n'abandi. [42] yasanze gukoresha ifumbire mvaruganda N ifumbire ya P byazamuye cyane umusaruro nubwiza bwimbuto. Nyamara, ingaruka zintungamubiri kumiterere ya YCH ntisobanutse, kandi ingamba zifumbire zo kuzamura ireme ryibikoresho byimiti ikeneye ubushakashatsi bwimbitse.Imiti y'ibyatsi yo mu Bushinwa ifite ibiranga “Ahantu heza hateza umusaruro, kandi ahantu hatameze neza hazamura ireme” [43]. Mugihe cyo guhinduranya buhoro buhoro kuva mwishyamba ukajya guhingwa YCH, aho ibimera byahindutse biva mubutayu bwumutse kandi butagira ubutayu buhinduka umurima urumbuka ufite amazi menshi. Gutura YCH ihingwa irarenze kandi umusaruro ni mwinshi, bifasha guhaza isoko. Nyamara, iyi miturirwa isumba iyindi yatumye habaho impinduka zikomeye muri metabolite ya YCH; niba ibi bifasha kuzamura ireme rya YCH nuburyo bwo kugera ku musaruro mwiza wa YCH binyuze mu ngamba zishingiye ku buhinzi busaba ubushakashatsi.Guhinga ahantu hatuwe ni uburyo bwo kwigana aho ibidukikije n’ibidukikije by’imiti y’imiti yo mu gasozi, bishingiye ku bumenyi bw’imihindagurikire y’igihe kirekire y’ibimera n’imihindagurikire y’ibidukikije [43]. Mu kwigana ibintu bitandukanye bidukikije bigira ingaruka ku bimera byo mu gasozi, cyane cyane aho umwimerere w’ibimera bikoreshwa nk’isoko ry’ibikoresho by’imiti nyabyo, ubwo buryo bukoresha igishushanyo mbonera cya siyansi no guhanga udushya tw’abantu kugira ngo duhuze imikurire n’imihindagurikire y’ibiti bivura Ubushinwa [43]. Uburyo bugamije kugera kuri gahunda nziza yo guteza imbere ibikoresho byiza byimiti. Guhinga ahantu hatuwe bigomba gutanga inzira nziza yumusaruro mwiza wa YCH nubwo hashingiwe kumiti ya farumasi, ibimenyetso byubuziranenge hamwe nuburyo bwo gusubiza ibintu bidukikije bidasobanutse. Kubera iyo mpamvu, turasaba ko igishushanyo mbonera cya siyansi n’ingamba zo gucunga imirima mu guhinga no kubyaza umusaruro YCH bigomba gukorwa hifashishijwe ibiranga ibidukikije biranga YCH yo mu gasozi, nk’ubutaka bwumutse, butarumbuka n’umucanga. Muri icyo gihe, hifujwe kandi ko abashakashatsi bazakora ubushakashatsi bwimbitse ku bikoresho bifatika n'ibimenyetso byiza bya YCH. Ubu bushakashatsi burashobora gutanga ibipimo ngenderwaho byiza kuri YCH, kandi bigateza imbere umusaruro mwiza wo mu rwego rwo hejuru niterambere rirambye ryinganda. -
Ibimera bya Fructus Amomi Amavuta ya massage Diffusers 1kg Ubwinshi Amomum villosum Amavuta yingenzi
Umuryango wa Zingiberaceae warushijeho kwitabwaho mubushakashatsi bwa allelopathique kubera amavuta akungahaye cyane hamwe n'impumuro yubwoko bwabanyamuryango. Ubushakashatsi bwibanze bwari bwerekanye ko imiti iva muri Curcuma zedoaria (zedoary) [40], Alpinia zerumbet (Pers.) BLBurtt & RMSm. [41] na Zingiber officinale Rosc. [42] yumuryango wigitoki bigira ingaruka za allelopathique kumera kwimbuto no gukura kwingemwe yibigori, salitusi ninyanya. Ubu bushakashatsi bwacu ni raporo yambere kubikorwa bya allelopathique yibikorwa bihindagurika biva kumuti, amababi, n'imbuto zikiri nto za A. villosum (umwe mubagize umuryango wa Zingiberaceae). Umusaruro w'amavuta y'ibiti, amababi, n'imbuto zikiri nto byari 0.15%, 0,40%, na 0,50%, byerekana ko imbuto zatanze amavuta menshi ahindagurika kuruta ibiti n'amababi. Ibintu nyamukuru bigize amavuta ahindagurika ava ku giti ni β-pinene, β-phellandrene na α-pinene, wari icyitegererezo gisa n’imiti minini y’amavuta y’ibabi, β-pinene na α-pinene (hydrocarbone ya monoterpene). Ku rundi ruhande, amavuta mu mbuto zikiri nto yari akungahaye kuri acetate ya bornyl na camphor (monoterpène ya ogisijeni). Ibisubizo byashyigikiwe nubushakashatsi bwa Do N Dai [30,32] na Hui Ao [31] uwari wamenye amavuta ava mu ngingo zitandukanye za A. villosum.
Habayeho raporo nyinshi kubikorwa byo guhagarika imikurire yibihingwa byingenzi mubindi binyabuzima. Shalinder Kaur yasanze α-pinene yo muri eucalyptus yahagaritse cyane uburebure bwumuzi no kurasa uburebure bwa virusi ya Amaranthus L. kuri 1.0 μL yibanze [43], n'ubundi bushakashatsi bwerekanye ko α-pinene yabujije imizi hakiri kare kandi itera kwangiza okiside mu mizi binyuze mu kongera amoko ya ogisijeni ikora [44]. Raporo zimwe zavuze ko β-pinene yabujije kumera no gukura kwatewe n’ibyatsi byo kwipimisha mu buryo butandukanye bitewe n’ibisubizo biterwa no guhungabanya ubusugire bwa membrane [45], guhindura ibinyabuzima bya biohimiki no kongera ibikorwa bya peroxidase na okiside ya polifenol [46]. P-Phellandrene yerekanye ko yabujije kumera no gukura kwa Vigna unguiculata (L.) Walp ku gipimo cya 600 ppm [47], mu gihe, ku gipimo cya mg / m3 250, camphor yahagaritse radicle no kurasa kwa Lepidium sativum L. [48]. Nyamara, ubushakashatsi bwerekana ingaruka za allelopathic ya bornyl acetate ni nto. Mu bushakashatsi bwacu, ingaruka za allelopathique ya β-pinene, acetate ya bornyl na camphor ku burebure bwumuzi yari ifite intege nke ugereranije n’amavuta ahindagurika usibye α-pinene, mu gihe amavuta y’ibabi, akungahaye kuri α-pinene, na yo yari phytotoxic kurusha ihindagurika rihuye. amavuta ava mumuti n'imbuto za A. villosum, ubushakashatsi bwombi bwerekana ko α-pinene ishobora kuba imiti yingenzi ya allelopathie nubu bwoko. Muri icyo gihe, ibisubizo byerekanaga kandi ko ibice bimwe na bimwe mu mavuta yimbuto bitari byinshi bishobora kugira uruhare mu gutanga umusaruro wa phytotoxique, ubushakashatsi bukeneye ubundi bushakashatsi mu gihe kizaza.Mubihe bisanzwe, ingaruka za allelopathique ya allelochemicals ni ubwoko bwihariye. Jiang n'abandi. yasanze amavuta yingenzi yakozwe na Artemisia sieversiana yagize ingaruka zikomeye kuri Amaranthus retroflexus L. kuruta kuri Medicago sativa L., Poa annua L., na Pennisetum alopecuroides (L.) Spreng. [49]. Mu bundi bushakashatsi, amavuta ahindagurika ya Lavandula angustifolia Mill. yabyaye impamyabumenyi zitandukanye zingaruka za phytotoxic kumoko atandukanye yibimera. Lolium multiflorum Lam. ni bwo bwoko bwakiriwe neza cyane, imikurire ya hypocotyl na radicle yabujijwe na 87.8% na 76.7%, ku kigero cy’amavuta ya 1 μL / mL, ariko gukura kwa hypocotyl kw ingemwe zimbuto ntibyagize ingaruka nke [20]. Ibisubizo byacu byerekanaga kandi ko hari itandukaniro ryo kwiyumvisha ibihindagurika bya A. villosum hagati ya L. sativa na L. perenne.Ibintu bihindagurika hamwe namavuta yingenzi yubwoko bumwe arashobora gutandukana mubwinshi na / cyangwa muburyo bwiza bitewe nuburyo imikurire, ibice byibimera nuburyo bwo gutahura. Kurugero, raporo yerekanaga ko pyranoide (10.3%) na β-caryophyllene (6,6%) aribyo bintu nyamukuru by’ibinyabuzima biva mu mababi ya Sambucus nigra, mu gihe benzaldehyde (17.8%), α-bulnesene (16,6%) na tetracosane (11,5%) byari byinshi mu mavuta yakuwe mu mababi [50]. Mu bushakashatsi bwacu, ibinyabuzima bihindagurika byasohowe nibikoresho bishya by’ibihingwa byagize ingaruka zikomeye za allelopathique ku bimera bipimishije kuruta amavuta yavomwe mu binyabuzima, itandukaniro mu gisubizo rifitanye isano rya hafi n’itandukaniro riri muri allelochemicique iri mu myiteguro yombi. Itandukaniro nyaryo riri hagati yimiterere ihindagurika hamwe namavuta bigomba gukorwaho ubushakashatsi mubushakashatsi bwakurikiyeho.Itandukaniro ryubwoko butandukanye bwa mikorobe hamwe nubuzima bwa mikorobe mubutaka bwubutaka bwongewemo amavuta ahindagurika byari bifitanye isano no guhatanira mikorobe ndetse ningaruka zose z'uburozi hamwe nigihe amavuta ahindagurika mubutaka. Vokou na Liotiri [51] yasanze gukoresha amavuta ane yingenzi (0.1 mL) kubutaka bwahinzwe (150 g) byatumaga guhumeka byubutaka bwubutaka, ndetse namavuta atandukanye mubigize imiti, byerekana ko amavuta yibimera akoreshwa nka karubone ningufu zitangwa na ibinyabuzima bibaho. Amakuru yavuye mu bushakashatsi bwakozwe yemeje ko amavuta yavuye mu gihingwa cyose cya A. villosum yagize uruhare mu kwiyongera kugaragara kw’ibinyabuzima by’ibihumyo by’ubutaka ku munsi wa 14 nyuma yo kongeramo amavuta, byerekana ko amavuta ashobora gutanga isoko ya karubone kuri byinshi ibihumyo. Ubundi bushakashatsi bwerekanye ubushakashatsi bwakozwe: mikorobe y’ubutaka yagaruye imikorere yambere na biomass nyuma yigihe gito cyo gutandukana kwatewe no kongeramo amavuta ya Thymbra capitata L. (Cav), ariko amavuta kumupanga mwinshi (amavuta 0,93 µL kuri garama yubutaka) ntabwo yemereye mikorobe yubutaka kugarura imikorere yambere [52]. Mu bushakashatsi burimo gukorwa, dushingiye ku isesengura rya mikorobe y’ubutaka nyuma yo kuvurwa n’iminsi itandukanye hamwe n’ubushakashatsi, twatekereje ko umuryango wa bagiteri w’ubutaka uzakira nyuma yiminsi myinshi. Ibinyuranye, microbiota ya fungal ntishobora gusubira muburyo bwambere. Ibisubizo bikurikira birashimangira iyi hypothesis: ingaruka zinyuranye z’amavuta menshi yibigize mikorobe ya mikorobe yubutaka bwagaragajwe nisesengura ryibanze ryakozwe (PCoA), kandi ubushakashatsi bwerekana ubushyuhe bwongeye kwemeza ko umuryango w’ibihumyo bigize ubutaka. bivuwe n'amavuta ya 3.0 mg / mL (ni ukuvuga 0,375 mg kuri garama y'ubutaka) kurwego rwubwoko bitandukanye cyane nubundi buvuzi. Kugeza ubu, ubushakashatsi bujyanye n'ingaruka zo kongeramo hydrocarbone ya monoterpene cyangwa monoterpène ya ogisijeni kuri mikorobe itandukanye y'ubutaka n'imiterere y'abaturage biracyari bike. Ubushakashatsi buke bwerekanye ko α-pinene yongereye ibikorwa bya mikorobe yubutaka hamwe nubwinshi bwa Methylophilaceae (itsinda rya methylotrophs, Proteobacteria) munsi yubushyuhe buke, bigira uruhare runini nkisoko ya karubone mubutaka bwumutse [53]. Mu buryo nk'ubwo, amavuta ahindagurika ya A. villosum igihingwa cyose, kirimo 15.03% α-pinene (Imbonerahamwe y'inyongera S1), biragaragara ko byongereye ubwinshi bwa Proteobacteria kuri 1.5 mg / mL na 3.0 mg / mL, ibyo bikaba byavugaga ko α-pinene ishobora kuba imwe mu nkomoko ya karubone ikomoka kuri mikorobe.Ibintu bihindagurika byakozwe ningingo zitandukanye za A. villosum byagize impamyabumenyi zitandukanye zingaruka za allelopathique kuri L. sativa na L. perenne, ibyo bikaba byari bifitanye isano rya bugufi nibigize imiti ibice bya villosum birimo. Nubwo imiti y’amavuta ihindagurika yemejwe, ibinyabuzima bihindagurika byasohowe na A. villosum ku bushyuhe bw’icyumba ntibizwi, bikeneye iperereza rindi. Byongeye kandi, ingaruka zifatika hagati ya allelochemicals nazo zikwiye kwitabwaho. Kubijyanye na mikorobe yubutaka, kugirango tumenye ingaruka zamavuta ahindagurika kuri mikorobe yubutaka byimazeyo, turacyakeneye gukora ubushakashatsi bwimbitse: kongera igihe cyo kuvura amavuta ahindagurika no gutandukanya ibintu bitandukanye mubigize imiti ya peteroli ihindagurika mubutaka. ku minsi itandukanye. -
Amavuta meza ya Artemisia capillaris ya buji hamwe nisabune ikora amavuta menshi ya diffuzeri amavuta yingenzi kubitsa urubingo.
Igishushanyo mbonera
Inyamaswa zagabanyijwemo amatsinda atanu yimbeba cumi nagatanu. Itsinda rishinzwe kugenzura nimbeba zicyitegererezo zashizwe hamweamavuta ya sesameiminsi 6. Imbeba zo kugenzura neza imbeba zapimwe hamwe na tableti ebyiri (BT, 10 mg / kg) muminsi 6. Amatsinda yubushakashatsi yavuwe 100 mg / kg na 50 mg / kg AEO yashonga mumavuta ya sesame muminsi 6. Ku munsi wa 6, itsinda ryigenzura ryavuwe namavuta ya sesame, andi matsinda yose yavuwe hamwe numuti umwe wa 0.2% CCl4 mumavuta ya sesame (10 ml / kg) nainshinge. Imbeba zahise zisiba nta mazi, kandi amaraso yakusanyirijwe mu mitsi ya retrobulbar; amaraso yakusanyijwe yashyizwe kuri 3000 ×gkuminota 10 yo gutandukanya serumu.Gutandukanya inkondo y'umurabyakozwe ako kanya nyuma yo gukuramo amaraso, kandi umwijima wavanyweho bidatinze. Igice kimwe cyicyitegererezo cyumwijima cyahise kibikwa kuri −20 ° C kugeza gisesenguwe, ikindi gice cyacukuwe kandi gishyirwa muri 10%formalinigisubizo; imyenda isigaye yabitswe kuri −80 ° C kugirango isesengure rya histopathologique (Wang n'abandi, 2008,Hsu n'abandi., 2009,Nie n'abandi., 2015).
Gupima ibipimo bya biohimiki muri serumu
Gukomeretsa umwijima byagereranijwe no kugereranyaibikorwa byimikorereya serumu ALT na AST ukoresheje ibikoresho byubucuruzi bijyanye bijyanye n'amabwiriza y'ibikoresho (Nanjing, Intara ya Jiangsu, Ubushinwa). Ibikorwa byimisemburo byagaragaye nkibice kuri litiro (U / l).
Gupima MDA, SOD, GSH na GSH-Pxumwijima homogenates
Uturemangingo twumwijima twahujwe hamwe na saline physiologique ikonje ku kigereranyo cya 1: 9 (w / v, umwijima: saline). Abahuje ibitsina bashizwemo (2500 ×gkuminota 10) gukusanya ndengakamere kubyemezo bizakurikiraho. Kwangirika kwumwijima byasuzumwe hakurikijwe ibipimo bya hepatike byurwego rwa MDA na GSH kimwe na SOD na GSH-Pxibikorwa. Aba bose biyemeje gukurikiza amabwiriza ku gikoresho (Nanjing, Intara ya Jiangsu, Ubushinwa). Ibisubizo kuri MDA na GSH byagaragaye nka nmol kuri proteine ya mg (nmol / mg prot), n'ibikorwa bya SOD na GSH-Pxbyagaragajwe nka U kuri proteine ya mg (U / mg prot).
Isesengura rya Histopathologique
Ibice byumwijima wabonye bishya byakosowe muri 10%paraformaldehydeigisubizo cya fosifate. Icyitegererezo noneho cyashyizwe muri paraffine, gicamo ibice 3-5 mm, bisizwe hamwehematoxylinenaeosin(H&E) ukurikije uburyo busanzwe, hanyuma burasesengurwa namicroscopi yoroheje(Tian n'abandi, 2012).
Isesengura mibare
Ibisubizo byagaragaye nkuburyo ± gutandukana bisanzwe (SD). Ibisubizo byasesenguwe hakoreshejwe gahunda y'ibarurishamibare SPSS Ibarurishamibare, verisiyo 19.0. Amakuru yakorewe isesengura ryibitandukanye (ANOVA,p<0.05) hakurikiraho ikizamini cya Dunnett na Dunnett ya T3 kugirango hamenyekane itandukaniro rishingiye ku mibare hagati yindangagaciro zamatsinda atandukanye. Itandukaniro rikomeye ryasuzumwe kurwego rwap<0.05.
Ibisubizo n'ibiganiro
Abagize AEO
Isesengura rya GC / MS, wasangaga AEO irimo ibice 25 byatoranijwe kuva min 10 kugeza 35, naho 21 bigizwe na 84% byamavuta yingenzi (Imbonerahamwe 1). Amavuta ahindagurika arimomonoterpenoids(80,9) Ugereranije nubundi bushakashatsi (Guo n'abandi., 2004), twasanze monoterpenoide nyinshi (80.90%) muri AEO. Ibisubizo byerekanye ko igice kinini cya AEO ari β-citronellol (16.23%). Ibindi bice byingenzi bigize AEO harimo 1.8-cineole (13.9%),kamfora(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3,99%),thymol(3.22%), namyrcene(2.02%). Guhindagurika mubigize imiti bishobora kuba bifitanye isano n’ibidukikije igihingwa cyahuye nacyo, nkamazi yubutaka, urumuri rwizuba, icyiciro cyiterambere kandiimirire.
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Amavuta meza ya Saposhnikovia divaricata ya buji nisabune akora amavuta menshi ya diffuser amavuta yingenzi kubitsa urubingo.
2.1. Gutegura SDE
Inkeri ya SD yaguzwe nk'icyatsi cyumye muri Hanherb Co (Guri, Koreya). Ibikoresho by'ibihingwa byemejwe na tagisi na Dr. Go-Ya Choi wo mu kigo cya Koreya gishinzwe ubuvuzi bw’iburasirazuba (KIOM). Icyitegererezo cyerekana inomero (nimero 2014 SDE-6) yashyizwe muri Herbarium yo muri Koreya y’umutungo kamere w’ibimera. Inkeri zumye za SD (320 g) zavanywemo kabiri hamwe na 70% Ethanol (hamwe na 2 h yagarutse) hanyuma ibiyikuramo noneho byibanda kumuvuduko muke. Decoction yarayungurujwe, lyofilize, ibikwa kuri 4 ° C. Umusaruro wibikoresho byumye biva mubikoresho bitangiye byari 48.13% (w / w).
2.2. Isesengura ryinshi-Imikorere ya Liquid Chromatografiya (HPLC) Isesengura
Isesengura rya Chromatografique ryakozwe hamwe na sisitemu ya HPLC (Amazi Co, Milford, MA, USA) hamwe na deteri ya Photodiode. Kubisesengura HPLC ya SDE, prima-O-glucosylcimifugin yaguzwe mu kigo cya Koreya gishinzwe guteza imbere inganda z’ubuvuzi gakondo (Gyeongsan, Koreya), nasec-O-glucosylhamaudol na 4′-O-β-D-glucosyl-5-O-methylvisamminol yitaruye muri laboratoire yacu kandi igaragazwa nisesengura ryerekanwa, cyane cyane NMR na MS.
Ingero za SDE (0.1 mg) zashongeshejwe muri 70% Ethanol (10 mL). Gutandukanya Chromatografique byakozwe hamwe na XSelect HSS T3 C18 inkingi (4,6 × 250 mm, 5μm, Amazi Co, Milford, MA, Amerika). Icyiciro kigendanwa cyari kigizwe na acetonitrile (A) na 0.1% acide acetike mumazi (B) kumuvuduko wa 1.0 mL / min. Porogaramu ya Multistep gradient yakoreshejwe kuburyo bukurikira: 5% A (0 min), 5-20% A (0-10 min), 20% A (10-23 min), na 20-65% A (23-40 min) ). Uburebure bwumurambararo bwerekanwe kuri 210-400 nm hanyuma bwandikwa kuri 254 nm. Ingano yo gutera inshinge yari 10.0μL. Ibisubizo bisanzwe byo kumenya chromone eshatu byateguwe kumurongo wanyuma wa 7,781 mg / mL (prim-O-glucosylcimifugin), 31.125 mg / mL (4′-O-β-D-glucosyl-5-O-methylvisamminol), na 31.125 mg / mL (sec-O-glucosylhamaudol) muri methanol kandi ikabikwa kuri 4 ° C.
2.3. Isuzuma ryibikorwa byo kurwanya ibicanwaMuri Vitro
2.3.1. Umuco w'akagari no kuvura icyitegererezo
RAW 264.7 ingirabuzimafatizo zabonetse mu cyegeranyo cy’umuco w’Abanyamerika (ATCC, Manassas, VA, Amerika) kandi gikura mu buryo bwa DMEM burimo antibiyotike 1% na 5.5% FBS. Ingirabuzimafatizo zashyizwe mu kirere cyuzuye cya 5% CO2 kuri 37 ° C. Mu gukangura ingirabuzimafatizo, uburyo bwasimbujwe uburyo bushya bwa DMEM, na lipopolysaccharide (LPS, Sigma-Aldrich Chemical Co, St. Louis, MO, USA) kuri 1μg / mL yongeyeho SDE ihari cyangwa idahari (200 cyangwa 400μg / mL) hiyongereyeho 24 h.
2.3.2. Kumenya Oxide ya Nitric (OYA), Prostaglandine E2 (PGE2), Tumor Necrosis Factor-α(TNF-α), na Interleukin-6 (IL-6) Umusaruro
Ingirabuzimafatizo zavuwe na SDE kandi zikangurwa na LPS kuri 24 h. NTA musaruro wasesenguwe no gupima nitrite ukoresheje reagent ya Griess ukurikije ubushakashatsi bwabanje [12]. Ibanga rya cytokine ikongora PGE2, TNF-α, na IL-6 byariyemeje gukoresha ibikoresho bya ELISA (sisitemu ya R&D) ukurikije amabwiriza yabakozwe. Ingaruka za SDE kuri OYA na cytokine byakozwe kuri 540 nm cyangwa 450 nm ukoresheje Wallac EnVision™umusomyi wa microplate (PerkinElmer).
2.4. Isuzuma ryibikorwa bya AntiosteoarthritisMuri Vivo
2.4.1. Inyamaswa
Imbeba z'umugabo Sprague-Dawley (zifite ibyumweru 7) zaguzwe na Samtako Inc. (Osan, Koreya) kandi zicumbikirwa mubihe byagenzuwe hamwe na 12-h yumucyo / umwijima kuri° C naUbushuhe. Imbeba zahawe indyo ya laboratoire n'amaziad libitum. Inzira zose zigeragezwa zakozwe hubahirijwe amabwiriza y’ikigo cy’igihugu cy’ubuzima (NIH) kandi zemezwa na komite ishinzwe kwita ku nyamaswa n’imikoreshereze ya kaminuza ya Daejeon (Daejeon, repubulika ya Koreya).
2.4.2. Kwinjiza OA hamwe na MIA mu mbeba
Inyamaswa zatoranijwe kandi zishyirwa mumatsinda yo kuvura mbere yo gutangira ubushakashatsi (kuri buri tsinda). Igisubizo cya MIA (3 mg / 50μL ya 0,9% saline) yatewe mu buryo butaziguye mu mwanya w’imbere y’amavi y’iburyo munsi ya anesthesia yatewe no kuvanga ketamine na xylazine. Imbeba zagabanyijwemo amatsinda ane: ) indomethacine- (IM-) itsinda ryavuwe (2 mg / kg) hamwe no guterwa MIA. Imbeba zatanzwe mu kanwa hamwe na SDE na IM icyumweru 1 mbere yo guterwa MIA ibyumweru 4. Igipimo cya SDE na IM cyakoreshejwe muri ubu bushakashatsi cyari gishingiye ku bakoreshejwe mu bushakashatsi bwabanje [10,13,14].
2.4.3. Ibipimo bya Hindpaw Gukwirakwiza Ibiro
Nyuma yo kwinjizwa kwa OA, impirimbanyi yumwimerere mubushobozi bwo gutwara ibiro bya hindpaws yarahungabanye. Ikizamini kidafite ubushobozi (ibikoresho bya Linton, Norfolk, UK) byakoreshejwe mugusuzuma impinduka zo kwihanganira ibiro. Imbeba zashyizwe mubyumba byapimwe. Imbaraga zo gutwara ibiro zashyizwe mu gihimba cy'inyuma zagereranijwe mu gihe cya 3 s. Ikigereranyo cyo kugabana ibiro cyabazwe nuburinganire bukurikira:15].
2.4.4. Ibipimo bya Serumu Cytokine Urwego
Ingero zamaraso zashyizwe kuri g 1.500 kuminota 10 kuri 4 ° C; noneho serumu yakusanyirijwe ikabikwa kuri −70 ° C kugeza ikoreshejwe. Urwego rwa IL-1β, IL-6, TNF-α, na PGE2 muri serumu byapimwe hifashishijwe ibikoresho bya ELISA biva muri R&D Sisitemu (Minneapolis, MN, USA) ukurikije amabwiriza yabakozwe.
2.4.5. Isesengura-Igihe Cyuzuye RT-PCR Isesengura
RNA yose yakuwe mu ngingo zifatanije zivi ikivi ikoresheje TRI reagent® (Sigma-Aldrich, St. Louis, MO, USA), ihindurwa-yandikwa muri cDNA na PCR-yongerewe hakoreshejwe ibikoresho bya TM One Step RT PCR hamwe nicyatsi cya SYBR (Ikoreshwa rya Biosystems , Ikirwa Kinini, NY, Amerika). PCR nyayo-nyayo yakozwe hakoreshejwe Sisitemu ya Biosystems 7500 Real-Time PCR (Ikoreshwa rya Biosystems, Grand Island, NY, USA). Urutonde rwa primer hamwe na probe-ikurikiranye byerekanwe kumeza1. Aliquots z'icyitegererezo cDNAs hamwe na GAPDH cDNA zingana zingana na TaqMan® Universal PCR ivanze irimo ADN polymerase ukurikije amabwiriza yabakozwe (Applied Biosystems, Foster, CA, USA). Imiterere ya PCR yari min 2 kuri 50 ° C, min 10 kuri 94 ° C, 15 s kuri 95 ° C, na min 1 kuri 60 ° C kuri cycle 40. Ubwinshi bwa gene yintego bwagenwe hakoreshejwe igereranya rya Ct (umubare wikigereranyo cyumubare wambukiranya imipaka hagati yuburyo bwongerewe imbaraga), ukurikije amabwiriza yabakozwe.
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Amavuta meza ya Dalbergia Odoriferae Amavuta ya Lignum ya buji nisabune akora diffuzeri yamavuta ya peteroli yamavuta mashya kubitsa urubingo.
Igiti cyimitiDalbergia odoriferaT. Ubwoko bwa Chen, nabwo bwitwaLignum Dalbergia odoriferae[1], ni ubwokoDalbergia, umuryango Fabaceae (Leguminosae) [2]. Iki gihingwa cyakwirakwijwe cyane mu turere dushyuha two muri Amerika yo Hagati no mu majyepfo, Afurika, Madagasikari, na Aziya y'Iburasirazuba n'Amajyepfo [1,3], cyane cyane mu Bushinwa [4].D. impumuro nzizaamoko, azwi ku izina rya “Jiangxiang” mu Gishinwa, “Kangjinhyang” mu kinyakoreya, na “Koshinko” mu biyobyabwenge by'Abayapani, yakoreshejwe mu buvuzi gakondo mu kuvura indwara z'umutima n'imitsi, kanseri, diyabete, indwara z’amaraso, ischemia, kubyimba , necrosis, ububabare bwa rubagimpande, nibindi [5-7]. By'umwihariko, uhereye ku myiteguro y'ibyatsi yo mu Bushinwa, habonetse inkwi z'umutima kandi zakunze gukoreshwa mu rwego rwo kuvanga imiti y’ubucuruzi mu kuvura umutima, harimo na Qi-Shen-Yi-Qi, ibinini bya Guanxin-Danshen, n'inshinge ya Danshen [5,6,8-11]. Nkabandi benshiDalbergiaamoko, iperereza rya phytochemiki ryerekanye ko habaho flavonoide yiganjemo flavonoide, fenol, na sesquiterpene mu bice bitandukanye by’iki kimera, cyane cyane ku bijyanye n’umutima [12]. Byongeye kandi, raporo zitari nke za bioactive kuri cytotoxic, antibacterial, antioxidative, anti-inflammatory, antithrombotic, antiosteosarcoma, antiosteoporose, na vasorelaxant nibikorwa byo guhagarika alpha-glucosidase byerekana ko byombiD. impumuro nzizaIbikomoka kuri peteroli hamwe na metabolite yayo ya kabiri ni umutungo wingenzi mugutezimbere imiti mishya. Icyakora, nta kimenyetso cyatanzwe cyerekana muri rusange ibihingwa. Muri iri suzuma, turatanga incamake yibice byingenzi bigize imiti nisuzuma ryibinyabuzima. Iri suzuma ryagira uruhare mu gusobanukirwa indangagaciro gakondo zaD. impumuro nzizanandi moko afitanye isano, kandi itanga umurongo ngenderwaho mubushakashatsi buzaza.
