Amavuta meza ya Artemisia capillaris ya buji hamwe nisabune ikora amavuta menshi ya diffuzeri amavuta yingenzi kubitsa urubingo.

Amavuta meza ya Artemisia capillaris ya buji nisabune ikora amavuta menshi ya diffuser amavuta yingenzi kubitsa urubingo rutandukanya Ibiranga Ishusho

ibisobanuro bigufi:

Igishushanyo mbonera

Inyamaswa zagabanyijwemo amatsinda atanu yimbeba cumi nagatanu. Itsinda rishinzwe kugenzura nimbeba zicyitegererezo zashizwe hamweamavuta ya sesameiminsi 6. Imbeba zo kugenzura neza imbeba zapimwe hamwe na tableti ebyiri (BT, 10 mg / kg) muminsi 6. Amatsinda yubushakashatsi yavuwe 100 mg / kg na 50 mg / kg AEO yashonga mumavuta ya sesame muminsi 6. Ku munsi wa 6, itsinda ryigenzura ryavuwe namavuta ya sesame, andi matsinda yose yavuwe hamwe numuti umwe wa 0.2% CCl4 mumavuta ya sesame (10 ml / kg) nainshinge. Imbeba zahise zisiba nta mazi, kandi amaraso yakusanyirijwe mu mitsi ya retrobulbar; amaraso yakusanyijwe yashyizwe kuri 3000 ×gkuminota 10 yo gutandukanya serumu.Gutandukanya inkondo y'umurabyakozwe ako kanya nyuma yo gukuramo amaraso, kandi umwijima wavanyweho bidatinze. Igice kimwe cyicyitegererezo cyumwijima cyahise kibikwa kuri −20 ° C kugeza gisesenguwe, ikindi gice cyacukuwe kandi gishyirwa muri 10%formalinigisubizo; imyenda isigaye yabitswe kuri −80 ° C kugirango isesengure rya histopathologique (Wang n'abandi, 2008,Hsu n'abandi., 2009,Nie n'abandi., 2015).

Gupima ibipimo bya biohimiki muri serumu

Gukomeretsa umwijima byagereranijwe no kugereranyaibikorwa byimikorereya serumu ALT na AST ukoresheje ibikoresho byubucuruzi bijyanye bijyanye n'amabwiriza y'ibikoresho (Nanjing, Intara ya Jiangsu, Ubushinwa). Ibikorwa byimisemburo byagaragaye nkibice kuri litiro (U / l).

Gupima MDA, SOD, GSH na GSH-Pxumwijima homogenates

Uturemangingo twumwijima twahujwe hamwe na saline physiologique ikonje ku kigereranyo cya 1: 9 (w / v, umwijima: saline). Abahuje ibitsina bashizwemo (2500 ×gkuminota 10) gukusanya ndengakamere kubyemezo bizakurikiraho. Kwangirika kwumwijima byasuzumwe hakurikijwe ibipimo bya hepatike byurwego rwa MDA na GSH kimwe na SOD na GSH-Pxibikorwa. Aba bose biyemeje gukurikiza amabwiriza ku gikoresho (Nanjing, Intara ya Jiangsu, Ubushinwa). Ibisubizo kuri MDA na GSH byagaragaye nka nmol kuri proteine ya mg (nmol / mg prot), n'ibikorwa bya SOD na GSH-Pxbyagaragajwe nka U kuri proteine ya mg (U / mg prot).

Isesengura rya Histopathologique

Ibice byumwijima wabonye bishya byakosowe muri 10%paraformaldehydeigisubizo cya fosifate. Icyitegererezo noneho cyashyizwe muri paraffine, gicamo ibice 3-5 mm, bisizwe hamwehematoxylinenaeosin(H&E) ukurikije uburyo busanzwe, hanyuma burasesengurwa namicroscopi yoroheje(Tian n'abandi, 2012).

Isesengura mibare

Ibisubizo byagaragaye nkuburyo ± gutandukana bisanzwe (SD). Ibisubizo byasesenguwe hakoreshejwe gahunda y'ibarurishamibare SPSS Ibarurishamibare, verisiyo 19.0. Amakuru yakorewe isesengura ryibitandukanye (ANOVA,p<0.05) hakurikiraho ikizamini cya Dunnett na Dunnett ya T3 kugirango hamenyekane itandukaniro rishingiye ku mibare hagati yindangagaciro zamatsinda atandukanye. Itandukaniro rikomeye ryasuzumwe kurwego rwap<0.05.

Ibisubizo n'ibiganiro

Abagize AEO

Isesengura rya GC / MS, wasangaga AEO irimo ibice 25 byatoranijwe kuva min 10 kugeza 35, naho 21 bigizwe na 84% byamavuta yingenzi (Imbonerahamwe 1). Amavuta ahindagurika arimomonoterpenoids(80,9) Ugereranije nubundi bushakashatsi (Guo n'abandi., 2004), twasanze monoterpenoide nyinshi (80.90%) muri AEO. Ibisubizo byerekanye ko igice kinini cya AEO ari β-citronellol (16.23%). Ibindi bice byingenzi bigize AEO harimo 1.8-cineole (13.9%),kamfora(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3,99%),thymol(3.22%), namyrcene(2.02%). Guhindagurika mubigize imiti bishobora kuba bifitanye isano n’ibidukikije igihingwa cyahuye nacyo, nkamazi yubutaka, urumuri rwizuba, icyiciro cyiterambere kandiimirire.

FOB Igiciro:US $ 0.5 - 9,999 / Igice

Min.Umubare w'Itegeko:100 Igice / Ibice

Ubushobozi bwo gutanga:10000 Igice / Ibice buri kwezi

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Indwara y'umwijima, indwara isanzwe iterwa navirusi ya hepatite, ubusinzi, imiti yica umwijima, ingeso mbi zimirire no guhumanya ibidukikije, ni impungenge kwisi yose (Papay n'abandi., 2009). Nyamara, kuvura iyi ndwara akenshi biragoye kubitanga kandi bigira ingaruka zifatika. Abashinwa gakondoimiti y'ibyatsi, ishingiye ku nyandiko nyinshi zikoreshwa mu kuvura indwara z'umwijima, ziracyakoreshwa cyane n'Abashinwa (Zhao n'abandi., 2014).Artemisia capillarisThunb.,Asteraceaenk'uko bitangazwa na Bencao Gangmu, inyandiko zizwi cyane mu buvuzi gakondo bw'Abashinwa, zakoreshejwe cyane nk'umuti wo gukuraho ubushyuhe, kuzamuradiuresishanyuma ukureho jaundice kandi yanakoreshejwe nk'uburyohe mubinyobwa, imboga, na paste kubera impumuro yihariye.A. capillarisbyafashwe nkubwoko bwimiti yabaturage nubushinwa nibiribwa byiyongera. Kubwibyo, habaye imbaraga nyinshi zo guteza imbere imiti y'ibyatsi, nkaA. capillaris, kuvura indwara y'umwijima.

Mu myaka yashize, imiti y'ibyatsi yarushijeho kwitabwaho no gukundwa no kuvura indwara z'umwijima kubera umutekano wazo no gukora neza (Ding n'abandi., 2012).A. capillarisbyagaragaye ko ifite ibikorwa byiza bya hepatoprotective ishingiye kuburyo bwa farumasi bugezweho (Han n'abandi., 2006). Nibikoresho byingenzi byubuvuzi mubushinwa kandi ni anti-inflammatory izwi cyane (Cha n'abandi., 2009a),kolera(Yoon na Kim, 2011), no kurwanya ibibyimba (Feng n'abandi, 2013)umuti w'ibyatsi.

Phytochemicalubushakashatsi bwerekanye amavuta yingenzi ahindagurika,coumarins, naflavonol glycosidekimwe n'itsinda ry'abantu batamenyekanyeaglyconesKuvaA. capillaris(Komiya n'abandi., 1976,Yamahara n'abandi., 1989). Amavuta ya ngombwa yaA. capillaris(AEO) nimwe mubintu nyamukuru bya farumasi ikora kandi itanga anti-inflammatory (Cha n'abandi., 2009a) hamwe na anti-apoptotic (Cha n'abandi, 2009b). Ariko, nka AEO nimwe mubintu byingenzi bigizeA. capillaris, ibikorwa bya hepatoprotective ibikorwa byingenzi bigizeA. capillarisbigomba gushakishwa.

Muri ubu bushakashatsi, ingaruka zo gukingira AEO kurikarubone tetrachloride(CCl4) -yizehepatotoxicityyasuzumwe nuburyo bwa biohimiki, nka hepatikeyagabanije glutathione(GSH),malondialdehyde(MDA) urwego,superoxide(SOD), naglutathione peroxidase(GSH-Px) ibikorwa, kimwe n'ibikorwa byaaminotransferase(AST) naalanine aminotransferase(ALT) muri serumu. Ingano y’imvune y’umwijima iterwa na CCl4 nayo yasesenguwe hifashishijwe ubushakashatsi bwakozwe na histopathologique, iherekejwe n’isesengura rya phytochemiki na GC - MS kugirango hamenyekane abagize AEO.